IonOptix FSI800-ION-FMI-100 system for synchronous measurement of myocardial cell contraction and calcium ion concentration
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- Product Description
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IonOptix systems enable rapid real-time recording and simultaneous measurement of calcium and contraction in cells. We are unique in that we provide both fluorescence photometric measurements and geometric measurements of individual cells. All individual system components can operate independently or as a complete integrated workstation. This allows for precise and synchronized data acquisition, allowing researchers to obtain real-time, synchronized data on cell length and sarcomere and calcium, superior to using conventional fluorescence systems.
Real-time measurement of some physiological indicators of cardiomyocytes and the micro-dynamic changes of ion concentration in the sample can be obtained. For example, for calcium ions, "calcium transients" can be obtained while the cell contraction amplitude, sarcomere length and mechanical changes of individual cells caused by changes in calcium ions, the relationship between mechanics and length, and isometric or isotonic contraction can be achieved. Data of many other functional indicators. Due to the characteristics of continuous contraction and relaxation of living cardiomyocytes, it is necessary to measure the dynamics of the sample edge under the visual sample state, which can more accurately observe the cell activity state in real time.
Ischemia-reperfusion injury: changes in Ca2+ and pH value in myocardial cytoplasm;
Changes of Ca2+ in myocardial cell mitochondria, screening of inotropic drugs and study of their mechanism;
Myocardial injury caused by H2O2: the role of Na+;
MDMA-induced myocardial damage: the role of ROS;
Heart failure model research: functional study of various calcium regulatory proteins;
Technical parameters:
1. Dual excitation light source system
1.1 Real-time measurement of fluorescent ions, such as measuring calcium, by calculating the absolute concentration of calcium in real time through the calcium ion change ratio
1.2 Sampling frequency 1000Hz.
2.CCD camera cell length acquisition system: used to measure cell length and sarcomere data.
2.1 Resolution: ≥774×490 pixel
2.2 Frame rate: 250HZ-500HZ;
3. Online temperature control system: used for liquid temperature control in cell experiments to maintain cell activity.
3.1 Two constant temperature modes: PWM [8W] or analog [2W]
3.2 Special cell tank for myocardial cells, equipped with drug delivery and perfusion port, field electrical stimulation electrodes, and temperature probe.
4. Data acquisition system (host) and acquisition and analysis software:
4.1 Data acquisition system:
4.2 25-pin light source control port;
4.3 ±5V four-channel A/D converter, dual-channel D/A converter;
5. Collection and analysis software:
5.1 One software controls and records, two contraction measurement modes: visual dynamic measurement of cell contraction length, synchronous measurement of sarcomere and length, seamless connection of data, real-time graphical data feedback;
5.2 Myocardial cell sarcomere measurement records the sarcomere length change data in real time;
5.3 Measurement of contraction/relaxation length of a single cell: maximum length of cell relaxation, minimum length of cell contraction, cell contraction amplitude, cell contraction amplitude ratio, maximum rate of cell contraction, maximum rate of cell relaxation
6. Field electrical stimulator: dedicated to electrical stimulation and pacing of living myocardial cells
6.1 Frequency range: 0.01-99Hz;
6.2 Pulse domain wide range: 0.4-24msec;
7. Photomultiplier tube:
7.1 Sampling frequency 1000Hz.
7.2 The signal amplification factor is greater than 1000 times, and the photoelectric conversion rate is greater than 20%.
7.3 The ideal detection ion concentration is 0.1xKd to 10xKd, and the minimum ion concentration detection limit is: 1 nM.References:
Cardiac resynchronization therapy restores sympathovagal balance in the failing heart by differential remodeling of cholinergic signaling.
DeMazumder, Deeptankar, et al. Circulation research 116.10 (2015): 1691-1699. DOI: 10.1161/CIRCRESAHA.116.305268
Mechanochemotransduction during cardiomyocyte contraction is mediated by localized nitric oxide signaling.
Jian, Zhong, et al. Science signaling 7.317 (2014): ra27. DOI: 10.1126/scisignal.2005046
neonatal Transplantation Confers Maturation of PSC-Derived Cardiomyocytes Conducive to Modeling Cardiomyopathy
gun-Sik Cho, Dong I. Lee, et al Cell Report:S2211-1247(16)31745-4User Unit:
Air Force Medical University
Guangzhou Medical University
China Academy of Chinese Medical Sciences
Zhongnan Hospital of Wuhan University
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